Different sequencing technologies are available at our lab, including an Illumina iSCAN, MiSeq, NextSeq, HiSeq2500, HiSeq4000 and NovaSeq6000. These technologies make it possible to rapidly perform whole-genome sequencing (WGS), targeted (re-)sequencing (e.g., exome sequencing), (targeted) bisulphite sequencing [5(h)mC-Seq], as well as RNA sequencing. These techniques can be applied on fresh frozen (tumor) tissue, FFPE-based (tumor) tissue and liquid biopsies. Via a single-cell accelerator initiative at VIB, we have early access to the newest single-cell multi-omics technologies (scRNA-seq, ATAC-seq, DNA-seq, CITE-seq, CROP-seq and spatial profiling), including the 10X Genomics technology.
All technologies and bioinformatics pipelines are accessible collaboratively (contact: Diether Lambrechts) or on a fee-for-service basis (https://www.genomicscore.be/). The 10X Genomics technology and Illumina NovaSeq system for the single-cell RNA sequencing experiments at UZ Leuven and KU Leuven is accessible via our VIB – KU Leuven Single Cell Core Facility (https://www.vibcancer.be/expertise-centers/single-cell-platform).